Leveraging the iReceptor Gateway to derive biological insights from TCR repertoire sequencing data

TCR repertoire diversity encompasses over 10⁸ – 10⁹ unique clonotypes per individual, revealed by next generation sequencing. Changes in TCR repertoire diversity provide potential insights into diseases, infection, drug efficacy and drug toxicity. However, the analysis and interpretation of TCR data is non-trivial, given that each experiment generates data for 100,000 to a million T-cell clonotypes. Parsing and annotation of such data require a sophisticated platform that combines high quality annotation, with tools to search and compare the experimental data with billions of sequences available in public databases. The iReceptor Gateway is a federated platform that sources data from multiple public and private databases to ensure seamless interrogation of experimental data, adding layers of information including real-world evidence to the clonotypic information generated by the researcher. The Gateway follows the protocols established by the Adaptive Immune Receptor Repertoire (AIRR) Community for the curation, analysis and sharing of antibody/B-cell and T-cell receptor sequences in the AIRR Data Commons. In this webinar, MedGenome scientists and the iReceptor Gateway team will give an overview of TCR data generation, analysis and how to leverage the iReceptor Gateway to annotate and interpret these data.

Speaker Profiles

MedGenome Inc.

Kushal Suryamohan

Director of Bioinformatics Services, MedGenome Inc., USA

Kushal Suryamohan is Director of Bioinformatics Services at MedGenome, and leads the commercial bioinformatics services team. He oversees the optimization as well as development of pipelines for NGS data analysis. Previously, he worked at Genentech where he focused on analyzing long read, optical map sequencing and chromatin capture technology data to generate high quality de novo genome assemblies. He was also actively involved in applying high throughput NGS-based functional genomic screens of oncogenes to characterize oncogene function in cancer. His work has led to multiple publications in high-impact journals including Nature, Nature Genetics and Developmental Biology. Kushal holds a PhD in Biochemistry and a Master’s Degree in Computer Science from the University of Buffalo, State University of New York.

iReceptor Gateway

Felix Breden

Scientific Director, iReceptor and iReceptor Plus, USA

Felix Breden is professor emeritus at Simon Fraser University. He was Chair of the AIRR Community Executive from 2015, when the AIRR Community was founded, until 2019. Felix still serves on the AIRR Community Executive, and the Common Repository and Diagnostics Working Groups. He is also Scientific Manager of iReceptor Plus, a European-Canadian Consortium that is working to expand the AIRR Data Commons and the iReceptor Gateway that provides access to AIRR-seq repertoires in the Commons.

Brian Corrie

Technical Director, iReceptor and iReceptor Plus, USA

Brian Corrie is the Technical Director for the iReceptor Project, the Technical Manager for the iReceptor Plus Project, and the co-chair of the AIRR Common Repositories Working Group. Brian has a background in computer science with a focus of data visualization, computational science, and scientific collaboration.