NGS Services

MedGenome offers an end-to-end solution for next-generation sequencing as a service for a wide range of sample types (DNA, RNA and specialized samples like T cells and B cells) as shown in Figure 1. The user has flexible options to use our services from end-to-end or simply request services at any step of the workflow. To learn more about our services click on the tabs in the workflow below.

Figure 1 : Workflow of the NGS Services provided at MedGenome for a wide range of sample types. Shown in this diagram, we highlight the steps taken to validate successful sample preparation, library generation, sequencing and data analysis for successful completion of a project. Click on each tab to learn more about the options available, steps followed and the QC reports generated.

Sample QC : Qubit, Gel and TapeStation
Library QC : TapeStation Bioanalyzer and Qubit
Data QC : Read orientation, quality distribution, base distribution and GC distribution for all reads

Overview of NGS services workflow
Sample Type DNA (WGS/WES/Targeted) WTS/Targeted/Small RNA (Transcriptome)
Extracted Nucleic Acid Minimum: 200 ng (WGS/WES) 50 ng (Targeted) Minimum: 250 pg (DV200 > 25%)
Frozen cell pellets Minimum 1 x 106 cells (ship on dry ice) Minimum 1 x 106 cells (ship on dry ice)
Frozen tissues 30 mg frozen tissue or 20 mg stabilized tissue (ship on dry ice) 30 mg frozen tissue or 20 mg stabilized tissue (ship on dry ice)
FFPE slides 3-6 slides (10 μM thick) 250 mm2 surface area 3-8 slides (10 μM thick) 250 mm2 surface area
Curls 5 curls (10 μM thick) 8 curls (10 μM thick)
Single-cell Fresh or cells in sample prep agent Fresh or cells in sample prep agent

Lower RNA & DNA amounts can be used for special projects

Service Type Kit Used
RNA (whole transcriptome)
Transcribed exonic regions
Small RNA
Illumina TruSeq RNA,
Takara SMARTer Pico V2,
Illumina TruSeq RNA,
Access, Stranded (no capture probes used)
Whole exome (WES) Agilent SureSelect Human All Exon V5/V6 kit
Agilent SureSelect XT Mouse
Agilent SureSelect + UTR X5
TruSeq Exome
Whole genome (WGS) Illumina TruSeq DNA PCR-Free kit
10X WGS Chromium linked read prep
Illumina TruSeq NANO
KAPA (no capture probes used)
MethylSeq Agilent Sure Select XT Methyl-Seq Target Enrichment kit
TCR sequencing 10X V(D)J, SMARTer TCR Profiling
Panel sequencing Custom design (Nimblegen, IDT)
Sample Type Illumina Sequencing Platform
WGS/WES/Targeted Illumina NovaSeq 6000, HiSeq 2500, HiSeq X
Whole-transcriptome profiling Illumina NovaSeq 6000, HiSeq 2500, MiSeq
TCR profiling Illumina MiSeq, NovaSeq 6000 (high throughput single-cell)

WGS/WES Germline variant calling Workflow

Somatic Variant Calling and Annotation Pipeline

Whole-Transcriptome Analysis Pipeline

TCR Sequencing

10X Single Cell Gene Expression Workflow

SMART-Seq Workflow

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