Single cell B-cell receptor sequencing is used to identify paired heavy and light chain combination for antibodies against targets of interest, thus generating a large repertoire of monoclonal antibodies more efficiently than the hybridoma technology. MedGenome’s HiTMab (High-throughput monoclonal antibody discovery) platform based on its proprietary single cell genomics technology enables identification of multiple antigen specific B cells against a target.
In this whitepaper, we discuss the features of HitMab in speeding up the discovery of validated antibodies.
Spatial transcriptomics is a transformative technology that allows visualization and quantitation of mRNA molecules
while preserving their spatial context within a tissue sample. Unlike traditional transcriptomics, spatial transcriptomics
enables visualization of gene expression patterns within their anatomical locations. Here, we provide an overview of
spatial transcriptomics and its diverse applications across various fields including developmental biology, neuroscience,
cancer research, and regenerative medicine. By enabling high-resolution mapping of gene expression pattern within
tissues, spatial transcriptomics revolutionizes our understanding of complex biological systems, paving the way for novel
discoveries in biology and medicine.
Traditional genomic research techniques often mask the inherent heterogeneity within complex biological systems by averaging data from millions of cells. This limitation hinders our understanding of cellular diversity and its implications for health and disease. Single-cell omics technologies have emerged as powerful tools, allowing researchers to dissect this complexity at an unprecedented resolution, analyzing the genome, transcriptome, proteome, and epigenome of individual cells.
MedGenome is at the forefront of this revolution, offering a comprehensive suite of single-cell omics services designed to empower researchers with unparalleled insights into cellular function and heterogeneity. From sample preparation to data analysis, our expert team provides end-to-end solutions tailored to your specific research needs.
Comprehensive genome sequencing and annotation are fundamental to understanding the biology of any organism. While short-read sequencing technologies have revolutionized genomics, they struggle to resolve complex genomic regions and repetitive elements, leading to fragmented assemblies and incomplete annotations. PacBio long-read sequencing technology overcomes these limitations, enabling the generation of highly contiguous and accurate de novo genome assemblies, even for complex genomes.
MedGenome offers a streamlined and comprehensive solution for de novo genome assembly and annotation. Leveraging our expertise in high molecular weight DNA and RNA, long-read sequencing, and bioinformatics pipelines we can partner with you to generate a reference-quality genome for use in your biological research.
Understanding the complete landscape of human genetic variation is crucial for advancing disease research, enabling precision medicine, and unraveling the complexities of human evolution. While short-read sequencing technologies have been instrumental in identifying single nucleotide variants (SNVs), they often fall short in detecting and characterizing complex structural variants (SVs) and variations in difficult-to-sequence regions. PacBio HiFi sequencing, with its long reads and high accuracy, overcomes these limitations, providing a comprehensive view of the human genome and its variations.
MedGenome offers a powerful solution for comprehensive human variant detection using PacBio HiFi sequencing. Our integrated workflow combines cutting-edge technology with expert bioinformatics analysis, enabling researchers to uncover the full spectrum of genetic variation, from single base changes to complex genomic rearrangements.
The transcriptome, comprising all RNA transcripts within a cell or organism, provides a dynamic snapshot of gene expression and regulation. Accurately characterizing the transcriptome is crucial for understanding cellular function, disease mechanisms, and developing novel therapeutic interventions. While short-read RNA sequencing has advanced transcriptomics, it suffers from limitations in identifying full-length transcripts, characterizing isoforms, and detecting novel transcripts. PacBio long-read sequencing technology overcomes these challenges by capturing full-length RNA molecules, enabling a comprehensive and accurate view of the transcriptome with unparalleled resolution.
MedGenome offers both bulk and single-cell full-length RNA sequencing solutions using PacBio technology, providing researchers with a comprehensive toolkit for unraveling transcriptome complexity across diverse biological applications.
The Whitepaper discusses TCR repertoire profiling using bulk input (from cells and RNA) using the SMARTerTCR Profiling Kit (Takara Bio USA Inc) and single-cell inputs using the SMARTer single-cell TCR Profiling kit and the Chromium Immune Profiling solutions (10X Genomics). We have tested and optimized the commercially available library preparation methods to work for a wide range of input types to obtain TCR alpha, beta, gamma & delta clonotypes (for bulk), and TCR alpha and beta clonotypes from single-cell inputs.
In this whitepaper, we present MedGenome’s NGS based workflows for profiling of the TCR repertoire: namely a) Bulk TCR profiling using: SMARTer TCR α /β Profiling Kit (Takara Bio USA Inc) and modifications to the protocol for Gamma/Delta TCR repertoire profiling, b) Single cell TCR Profiling using: 10X Genomics Chromium Immune Profiling solutions, and Takara single-cell TCR sequencing kits. We also present an overview of the types of samples we have processed in-house and application.
This BCR Whitepaper discusses MedGenome’s capability to provide unparalleled sequencing solutions to analyze B-cell receptor (BCR) repertoires from human or mouse species. We utilize the Takara SMARTer BCR Profiling Kit that leverages SMART (Switching Mechanism at 5′ End of RNA Template) technology coupled with 5′ RACE approach, for unbiased gene and clonal amplification of BCR repertoire sequences.
Our solutions can help in:
At MedGenome, we provide BCR repertoire profiling from bulk input (from cells & RNA) using the SMARTer BCR Profiling Kit (Takara Bio USA Inc) and single-cell inputs using the Chromium Immune Profiling solutions (10X Genomics). The sample types we have validated include frozen PBMCs, hybridoma and spleen. We follow the manufacturers recommended guidelines for our quality control at every step of the process and provide customers with accurate QC reports. In addition, we support experimental design and basic and advanced analysis.
Cancer immunotherapy is now established as a major therapeutic modality, and 70% of all cancer patients are estimated to receive some form of immunotherapy treatment as a part of their disease control by 2025. Cancer immunotherapy drugs elicit their anti-tumor immune response in a subset of the treated patients by activating CD8 T-cells and provide sustainable and long-lasting benefit in a few. Recently significant efforts have been devoted to understanding the factors that influence response to immuno-therapy or contribute to the development of resistance to therapy. While it is appreciated that many different tumor cell- intrinsic and extrinsic features, including the tumor microenvironment, driver gene mutations, host genetics, microbiome and environmental factors modulate response to immune checkpoint inhibitors [1], the tumor microenvironment ecosystem could be a major contributor in regulating response to immunotherapy and development of resistance [2,3].
The TSO 500 portfolio consists of two assays which enable precision oncology through pan-cancer comprehensive genomic profiling (CGP) for solid tumors from either blood or tissue biopsy samples.
Illumina TSO 500 and TSO 500 ctDNA use a target enrichment (~2 Mb panel) workflow enhanced with unique molecular
indices (UMIs), targeting 523 cancer-relevant genes from tissue and/or liquid biopsy samples to interrogate for various cancer types (Figure 1). MedGenome employs proprietary bioinformatic workflows coupled to Dragen and Illumina Connected Insights for data analysis, providing high sensitivity and specificity to deliver a comprehensive cancer genomic profile for your samples.
Epigenetic mechanisms play an important role in regulating gene expression. Advances in high-throughput sequencing technologies has enabled genome-wide studies to map epigenetic landscape. Epigenetic studies are now routinely carried out using bulk cell/tissue samples as well as single cell samples. At MedGenome, we have effectively executed and delivered numerous projects on epigenetics studies. We provide an end-to-end solution for Single Cell ATAC Seq, Single Cell Multiome ATAC + Gene Expression, CUT & RUN, BULK ATAC sequencing services and bioinformatics analysis pipelines.
Spatial analysis has become indispensable in deciphering the complexities of biological systems. Delve deeper into our comprehensive spatial transcriptomics solutions to uncover valuable insights into gene function, tissue microenvironments, and beyond.
The landscape of drug discovery is marked by significant challenges, with drug efficacy being a primary reason for the failure of clinical trials and the rejection of drug candidates by regulatory bodies. Leveraging genetic data during drug discovery can markedly increase the chances of developing successful drug candidates and achieving clinical trial success. Understanding genetic variants associated with diseases is pivotal in selecting effective drug targets, paving the way for innovative therapies and improving the overall success rate in drug development.
MedGenome is a global multiomics solutions provider dedicated to advancing your research. Our state-of-the-art facility in California delivers the highest quality omics data with industry leading turnaround times.
Our solutions are supported by Illumina NovaSeq X+, 6000, MiSeq, and PacBio Revio sequencers. You can take advantage of our end-to-end solutions, including library prep, sequencing, and analysis, or you can submit premade libraries for sequencing only services.
Current genetic datasets often lack diversity and do not represent the global population accurately, which can create challenges in the drug development process. South Asians, making up 25% of the world’s population, are particularly valuable for enriching current datasets.
Due to its size, studying populations from South Asia, especially when researching rare diseases, can expedite recruitment for research studies. Additionally, the high levels of endogamy and consanguinity in South Asia make this population extremely unique, facilitating the study of homozygous gene function, which accelerates biomarker discovery in drug development.
MedGenome’s diagnostics business has amassed a significant dataset of South Asian patients across various diseases. Additionally, MedGenome has access to the largest network of hospitals, clinicians, and patients in India, for genomic research across numerous disease areas. By partnering with MedGenome, interesting and pertinent datasets can be built through prospective cohorts, seeded cohorts, or by mining our diagnostic databases.
Long-read sequencing provides highly accurate, comprehensive data by reading longer segments of DNA, enabling deeper insights into complex genomic regions. MedGenome provides end-to-end solutions for various applications, including:
Multiomics Services
Sequencing Services
Research & Pharma Solutions
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