It is estimated that there are approximately 1010–1011 B cells in a human adult. A BCR is composed of two identical heavy chains generated by recombination of V, D and J segments and two identical light chains generated by recombination of V and J segments. Human BCRs undergo recombination at these variable regions, generating a large diverse receptor profile for each cell. Utilizing targeted next-generation sequencing of these variable regions, can provide deeper insights into B-cell differentiation, BCR somatic hypermutation, class switching, and antigen specificity. Major applications of BCR repertoire profiling include diagnostic biomarker discovery, tracking known repertoire sequences and disease diagnosis.
Library preparation services using inputs from B cells, blood and total RNA with high quality and varying input
Human B-cell Receptor Profiling service at MedGenome Inc.
In this whitepaper, we discuss MedGenome's capability to provide sequencing solutions for analyzing B-cell receptor (BCR) repertoires from human or mouse species. We utilize the Takara SMARTer BCR Profiling Kit that leverages SMART (Switching Mechanism at 5' End of RNA Template) technology coupled with 5' RACE approach, for unbiased gene and clonal amplification of BCR repertoire sequences.
Our solutions can help in:
Human Bulk BCR Profiling Technical Sheet
At MedGenome, we provide BCR repertoire profiling from bulk input (cells & RNA) using the SMARTer BCR Profiling Kit (Takara Bio USA Inc) and single-cell inputs using the Chromium Immune Profiling solutions (10X Genomics). The sample types we have validated include frozen PBMCs, hybridoma and spleen. We follow the manufacturers recommended guidelines for our quality control at every step of the process and provide customers with accurate QC reports. In addition, we support experimental design and basic and advanced analysis.