NGS Services

Epigenomic Profiling

Epigenomic studies have a broader influence owing to their interest in deeper understanding of the underlying cause behind phenotypic changes for the similar genotypes. Studying the DNA, histone modifications and DNA methylation and other chromatin components of interest is a critical factor in epigenomic profiling.

CUT&Tag is a novel tool aiding in chromatin profiling. Compared to ChipSeq which requires a large amount of starting material with a poor signal-to-noise ratio, Cut&Tag can be a highly efficient technique which can provide high-resolution sequencing libraries for epigenomic profiling.

CUT&Tag assays take advantage of a Tn5 transposase that is fused with protein A, which directs the enzyme to the antibody bound target on chromatin. The Tn5 transposase is pre-loaded with sequencing adapters (generating an assembled protein A-Tn5 adapter transposome) to carry out antibody-targeted tagmentation. Adding Mg2+ activates the Tn5 transposase and results in adapter flanked fragments of DNA cut near the protein of interest. CUT&Tag can be used to profile the chromatin landscape at a single cell resolution with higher efficiency and lower sequencing depths than other available methods.

At MedGenome, we have tested and optimized the laboratory protocol and bioinformatics analysis pipeline to offer an end-to-end CUT&TAG service for epigenomic profiling.

Highlights of our Services

MedGenome’s CUT&Tag Epigenomic profiling workflow

Sample requirements for CUT&Tag analysis

  • Fresh or frozen cells/tissue
  • 50 k-500 k viable cells per reaction, including a negative control
  • Primary antibody against histone mark or transcription factor
  • Secondary antibody (species-specific)
  • Shipping- cryopreserved cells and all antibodies should be shipped on dry ice
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