Single Cell Research

Single cell analysis has become a gold standard application in both basic as well as translational research. Several approaches are now available that capture the maximal transcript diversity in a given cell and allowing for multi-model analysis strategies to generate meaningful insights. MedGenome has adopted several commercially available platforms for single cell analysis.

Discover Your Application

Solutions for fresh or frozen cell suspensions, isolated cells in 96 well plates or methanol fixed cells

Gene Expression
Analysis

  • Explore full length, 3' or 5' gene expression data for cellular heterogeneity, biomarker discovery and more
  • Combine gene expression with cell surface protein data for a multiomic look into rare cell populations or novel subsets

Immune Profiling
Analysis

  • Discover full length paired sequences for T and B cells repertoire analysis
  • Perform multiomic analysis with gene expression and immune repertoire data combined with cell surface protein data to look at functional states and subsets of immune cells

Epigenetic
Analysis

  • Detect chromatin accessibility on a single cell level
  • Analyze chromatin accessibility plus gene expression simultaneously

Service Highlights

  • Support with experimental design and selection of appropriate single cell workflow
  • Process single cell samples for a wide range of sample types and input amounts
  • End-to-end solutions for sample QC, viability assessment, single cell library preparation, sequencing and analysis
  • Advanced analysis offering includes custom visuals and publication ready figures for improved data interpretation

 

 

BioFX Single Cell Analysis

  • Streamlined pipeline for fast & scalable analysis
  • Customized reporting
  • Interactive reports
  • Publication-ready figures
  • Improve data interpretation with support from MedGenome's team of experts

 

Explore Analysis Reports

BioFX's single cell analysis platform was built using state-of-the-art analysis tools for gene expression, T-cell and B-cell sequencing data. Accomplish multiomic analysis by interrogating gene expression and VDJ sequencing data simultaneously.

Advanced
QC metrics

Differential Gene Expression
& Heatmap Visualization

Interactive tSNE plots with cell type & cluster information

 

Ordering and Data Delivery

MedGenome's customer portal allows for easy sample submission and sample tracking during lab processing. Data QC, raw data and interactive analysis reports are delivered securely through customer portal.

 

White Papers and Technical Resources

Single Cell PLATE Seq Solutions at MedGenome

In this white paper we discuss a robust and cost-effective solution for generating single cell RNA seq data from FACS sorted cells using the full-length SMART seq chemistry. This approach is useful when there are limited cells available, or larger cells that diminish capture efficiencies using droplet-based approaches. We have validated this approach and find that we can identify cellular heterogeneity and identify cell types from few hundred cells.

A platform-agnostic approach to single-cell genomic applications

Single cell genomics is a powerful approach to uncover cellular heterogeneity and understand molecular mechanisms of development and disease in normal and disease tissues. However, isolating or capturing a sufficient number of single cells to be able to answer the research question of interest is challenging. MedGenome has several options for single cell NGS processing. In this whitepaper, we highlight various workflows to help decide the best approach for your samples and scientific questions.

 

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