Single cell analysis has become a gold standard application in both basic as well as translational research. Several approaches are now available that capture the maximal transcript diversity in a given cell and allowing for multi-model analysis strategies to generate meaningful insights. MedGenome has adopted several commercially available platforms for single cell analysis.
Solutions for fresh or frozen cell suspensions, isolated cells in 96 well plates or methanol fixed cells
BioFX's single cell analysis platform was built using state-of-the-art analysis tools for gene expression, T-cell and B-cell sequencing data. Accomplish multiomic analysis by interrogating gene expression and VDJ sequencing data simultaneously.
Differential Gene Expression
& Heatmap Visualization
Interactive tSNE plots with cell type & cluster information
Single Cell PLATE Seq Solutions at MedGenome
In this white paper we discuss a robust and cost-effective solution for generating single cell RNA seq data from FACS sorted cells using the full-length SMART seq chemistry. This approach is useful when there are limited cells available, or larger cells that diminish capture efficiencies using droplet-based approaches. We have validated this approach and find that we can identify cellular heterogeneity and identify cell types from few hundred cells.
A platform-agnostic approach to single-cell genomic applications
Single cell genomics is a powerful approach to uncover cellular heterogeneity and understand molecular mechanisms of development and disease in normal and disease tissues. However, isolating or capturing a sufficient number of single cells to be able to answer the research question of interest is challenging. MedGenome has several options for single cell NGS processing. In this whitepaper, we highlight various workflows to help decide the best approach for your samples and scientific questions.