While single cell sequencing can provide valuable insights into the complexity and heterogeneity of the cell types in the context of a tissue or tumor, there are challenges that exist that one needs to consider to be able to extract meaningful data from a single cell experiment. Key challenges during the workflow starts with preparation of single cell suspensions with good cell viability, efficiently capturing the diverse cell types in a mix via the appropriate cell capture method, and additional complexities with the quality of the data generated in a single cell experiment. Due to the tremendous utility of single cell gene expression in both basic as well as translational research, several approaches are now available for optimal isolating & scaling single cells isolation, preparing sequencing ready libraries that capture the maximal transcript diversity in a given cell, and analysis strategies to generate meaningful information from the data. At MedGenome, we have adopted several of the commercially available platforms for single cell analysis including the 10x Genomics Chromium platform and the SMART-Seq chemistries by Takara. As part of our service workflow we inspect the cell suspensions for viability and assess the quality of the sample prior to proceeding with the library preparation. We follow the manufacturer’s recommended guidelines for our quality control in every step of the process and provide customers with accurate QC reports.
Highlights of our services are:
Support with experimental design and selection of appropriate single cell workflow
Process single cell samples for a wide range of sample types
Process single cell sequencing libraries from live and fixed tissues
Provide end to end solution in library preparation, sequencing and bioinformatics analysis