RNA SEQUENCING SERVICES

Decode molecular mechanisms through high-quality gene expression profiling

Comprehensive RNA Sequencing solutions

Cost efficient gene expression data from both good and poor quality RNA samples

MedGenome offers flexible library preparation options based on RNA quality and quantity allowing researchers to generate optimal quality data from their samples. We are able to work with RNA from all sources such as FFPE or low cell counts by leveraging highly optimized workflows and a consultative approach with customers to make informed decisions.

MedGenome Sequencing workflow

RNA sequencing services

Types of RNA sequencing services offered by MedGenome

Library Prep Services Assay Type Stranded Type Starting Material Input Amount
TruSeq Stranded mRNA mRNA Seq Yes RNA 100 ng - 1 μg RNA
Illumina Stranded mRNA mRNA Seq Yes RNA 25 ng - 1 μg RNA
Takara SMART-Seq V4 mRNA Seq No RNA, Cells 10 pg - 10 ng RNA, 1 - 1,000 cells
TruSeq Stranded Total RNA Total RNA Yes RNA 100 ng - 1 μg RNA
Pico V2 / V3 Total RNA Yes RNA 250 pg - 10 ng RNA
SMART-Seq Stranded Total RNA Yes RNA, Cells 10 pg - 10 ng RNA, 1 - 1,000 cells

RNA sequencing services

Our key features include

MedGenome Cost Efficient Service
Cost efficiency
MedGenome Fast turnaround time
Fast turnaround time (2 weeks)
MedGenome Dedicated customer portal
Dedicated customer portal with seamless customer experience
MedGenome Bioinformatics Consultation
QC & Bioinformatics consultation
Tumor Microenvironment Analysis
Tumor heterogeneity and immune infiltration
Explore tumor heterogeneity and immune infiltration by leveraging our proprietary algorithm

A genomics-based approach to analyze the tumor microenvironment can provide unique insights beyond IHC and FACS methods. OncoPeptTUME deeply interrogates RNA-Seq data sets to produce high resolution mapping of the tumor microenvironment using proprietary cell type specific gene expression signatures. OncoPeptTUME provides a critical assessment of the inflammatory microenvironment that modulates T-cell infiltration and its anti-tumor activities to enable better prediction of treatment response and efficacy.

The OncoPepTUME pipeline quantifies the relative infiltration of innate and adaptive immune cells (CD8+, CD4+, T cells, B cells, Treg cells, NK cells, Macrophages, Monocytes, Neutrophils) with proprietary immune cell type-specific gene expression signatures. Identify biomarkers for patient selection, predict likelihood of response to checkpoint inhibitor therapies and monitor durability of response.

Our patented OncoPeptTUME algorithm analyzes RNA-Seq data to produce a high-resolution mapping of the tumor microenvironment using proprietary cell type specific gene expression signatures. This provides a critical assessment of the inflammatory microenvironment that modulates T-cell infiltration and its anti-tumor activities to enable better prediction of treatment response and efficacy.

OncoPeptTUME Workflow Highlights

  • Requires low sample input from FFPE or fresh frozen tissues compared to IHC or FACS.
  • High throughput and robust workflow at significantly lower cost than IHC and FACS.
  • Provides an accurate representation of the tumor microenvironment at a cellular, molecular and biochemical level.
  • Shorter analysis time with high information content and less variability in data.
  • Can be customized to fit cancer immunotherapy project needs and tailored to perform in preclinical and clinical settings.
  • Continuously updated to include new insights from cancer immunotherapy basic and translational research.
Cancer Immunity Cycle

Service highlights

RNA Sequencing Applications

MedGenome Pre-Clinical Applications
Pre-Clinical Applications
 
  • Discover biomarkers for patient stratification.
  • High resolution mapping of gene expression in the tumor and stromal compartments.
MedGenome Clinical Research / Clinical Applications
Clinical Research / Clinical Applications
  • Discover biomarkers for patient selection.
  • Monitor durability of response.
  • Accurate quantification of biomarkers before and after drug treatment.
MedGenome Drug Post Approval Applications
Drug Post Approval Applications
  • Analyze patient outcome.
  • Evaluation of drug resistance.
  • Discover novel drug targets to increase efficacy and reduce toxicity.

Resources

White papers and technical resources

Resource Video
Video
RNA Sequencing Walkthrough
Whitepaper
Whitepaper
OncoPeptTUMETM — A novel in-silico approach to model the tumor microenvironment and predict treatment efficacy and long-term survival benefits for immunotherapy applications

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how to order

Starting a project with MedGenome

MedGenome's customer portal allows for easy sample submission and sample tracking during lab processing. Using our portal ensures data QC, raw data and interactive analysis reports are delivered securely.

Our process is simple

Request a quote Request a quote
We create your project file We create your project
Upload sample details Upload sample details
Submit documents and ship samples Submit documents and ship samples
We process and analyze your samples We process and analyze your samples
Your data is delivered to you Your data is delivered to you

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