Immuno-Oncology Solutions

BCR Repertoire Analysis

B-cells or B Lymphocytes are a vital component of the adaptive immune system. They are part of the humoral immunity and are responsible for secreting antibodies. B-cell receptors (BCRs), a membrane bound cell surface receptor bind to a specific antigen to initiate an immune response. BCRs are assembled by genetic rearrangement and somatic recombination of vast immunoglobulin gene segments and are constantly diversified to specifically bind exogenous antigens and endogenous host responses. Therefore, it becomes crucial to understand the role of BCRs in immune response regulation both in physiological and pathological conditions. B-cell dysregulation can lead to various diseases specifically:

Cancer Auto-immune Diseases Graft-versus-host diseases
  • Non-Hodgkin’s lymphoma (NHL)
  • Hodgkin’s lymphoma (HL)
  • Advanced stage follicular lymphoma
  • Lymphocytic leukaemias (CLL or SLL)
  • Burkitt’s lymphoma
  • Diffuse large B-cell lymphoma
  • Mantle Cell lymphoma
  • Marginal Zone lymphomas
  • Waldenstrom's macroglobulinemia
  • Hairy Cell leukemia
  • Primary CNS Lymphoma
  • Primary Intraocular lymphoma
  • Multiple sclerosis (MS)
  • Rheumatoid arthritis (RA)
  • Systemic lupus erythematosus (SLE)
  • Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis
  • Crohn’s disease
  • Behçet’s disease
Many studies have indicated the activation of B cell signaling pathways is evident in chronic graft-versus-host disease (cGVHD).

It is estimated that there are approximately 1010–1011 B cells in a human adult. A BCR is composed of two identical heavy chains (generated by recombination of V, D and J segments), and two identical light chains (generated by recombination of V and J segments). Human BCR undergo recombination at variable region of three gene segments of the immunoglobulin heavy chain locus (V, D, J) and two gene segments of immunoglobulin light chain locus (V, J).

Next-generation BCR sequencing can provide deeper insights into various dimensions such as B-cell differentiation, BCR somatic hypermutation, class switching, and antigen specificity.

Major Applications of BCR Repertoire Profiling

Highlights of our services are:

  • Flexibility in input & wider dynamic range for assays
    • Per cell more copies of transcripts vs genomic DNA ( several copies vs 2 copies for genomic DNA)
    • Higher sensitivity in detecting in lower frequency BCRs
    • Ease in detecting BCR transcripts from low quantity of input
  • Accurate representation of expressed BCRs vs non-functional BCRs ( stop codons at the RNA level can be ruled out upon analysis )
  • Ability to extract BCR and other expression information ( transcriptome for functional state of B-cells )
  • Reduced Amplification bias
  • Improved ability to call novel and low frequency clonotypes

End-to-End Complete Service – BCR Workflow

BCR Library Prep, Sequencing, and Analysis Service Offerings

Source Library Prep Options Analysis Offering
  • Cells
  • Purified Total RNA
  • Takara SMARTer BCR IgG H/K/L Profiling Kit
    (Human, Mouse)

Standard Analysis

  • MiXCR Output file - Clonotype Frequency, Gene and Amino Acid Sequences
  • V J Gene Usage and Circos Plots, Spectratype Plot
  • Custom figures available upon request

In addition to the BCR Seq services, MedGenome Inc. will also support the development of new diagnostic experimental design and advanced bioinformatics analysis.

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