Molecular Biology Services

Cloning Services

  • Cloning of genes from genomic DNA templates or cDNA libraries
  • Traditional cloning of inserts by PCR and digestion, ligation.
  • DNA sub-cloning from entry to destination vector.
  • Cloning of fusion genes by using seamless or Gibson DNA assembly techniques.
  • Knock-out / Knock-in Vector constructs for CRISPR-Cas9 genome editing.
  • Site-directed Mutagenesis such as single / multiple point mutations, insertions, deletions.
  • E. coli expression constructs with multiple choice of promoters (T7, tac, pho, rhamnose) and fusion tags (His, GST, MBP, Sumo)

Regular PCR-Insert Cloning

PCR Amplification of specific DNA fragments from genomic DNA / cDNA libraries and cloning into a standard vector or into the preferred vector of customer.

DNA sub-cloning

Restriction digestion of DNA inserts and sub-clone into specific vectors of interest, viz. for in vivo protein expression, for construction of hybrid genes, etc.

Site-directed Mutagenesis

Design and synthesis of mutagenic primers, mutagenesis reaction setup and Sanger sequencing to confirm desired DNA mutations.

Multi-DNA fragments Assembly and Cloning

Design of modular DNA fragments and construction of combinatorial gene variants using DNA assembly techniques.

Cloning in E. coli expression vectors

Customers can choose from the varied promoter types and fusion tags; and get their target genes cloned into these engineered vectors (refer the schematic representation image). These clones are ideal for protein over-expression studies in E. coli.

Cloning of CRISPR-gRNA constructs and HR donor vectors

Design of gRNAs, synthesis and cloning into any preferred vector of the customer. Design and clone the homology arms into donor plasmid for the knock-out, knock-in, and other CRISPR-Cas9 gene-editing modifications.

Examples of Vector Reconstructs

Vector features:

1. Vector ends with 3’ T-overhangs
2. Ampicillin resistance marker
3. pUC ori of replication

Vector features:

4. 5’ and 3’ homology arms
5. MCS with XcmI restriction site for TA cloning
6. EGFP with p10 promoter

Constructs made with pUVR vector:
Clone ID Construct Name Gene Product
SGP.878pUVR-Ac.ORF-02Ac.Orf-2
SGP.879pUVR-Ac.ORF-03conotoxin-like peptide
SGP.881pUVR-Ac.ORF-05Ac.Orf-5
SGP.882pUVR-Ac.ORF-06late expression factor 2
SGP.884pUVR-Ac.ORF-08major occlusion body protein
SGP.886pUVR-Ac.ORF-10protein kinase
SGP.888pUVR-Ac.ORF-12Ac.Orf-12
SGP.889pUVR-Ac.ORF-13Ac.Orf-13
SGP.890pUVR-Ac.ORF-14late expression factor 1
SGP.891pUVR-Ac.ORF-15ecdysteroid UDP-glucosyl transferase
SGP.893pUVR-Ac.ORF-17Ac.Orf-17
SGP.894pUVR-Ac.ORF-18Ac.Orf-18
SGP.896pUVR-Ac.ORF-20actin rearrangement inducing factor
SGP.897pUVR-Ac.ORF-21actin rearrangement inducing factor
SGP.901pUVR-Ac.ORF-25ssDNA binding protein
SGP.902pUVR-Ac.ORF-26Ac.Orf-26
SGP.903pUVR-Ac.ORF-27apoptosis inhibitor, IAP-1
SGP.904pUVR-Ac.ORF-28late expression factor 6
SGP.905pUVR-Ac.ORF-29Ac.Orf-29
SGP.906pUVR-Ac.ORF-30Ac.Orf-30
SGP.907pUVR-Ac.ORF-31superoxide dismutase
SGP.909pUVR-Ac.ORF-33putative histidinol-phosphatase
SGP.910pUVR-Ac.ORF-34Ac.Orf-34
SGP.912pUVR-Ac.ORF-36nuclear matrix associated phosphoprotein
SGP.914pUVR-Ac.ORF-38Ac.Orf-38
SGP.915pUVR-Ac.ORF-39Ac.Orf-39
SGP.916pUVR-Ac.ORF-40transcription regulator, p47
SGP.920pUVR-Ac.ORF-44Ac.Orf-44
SGP.921pUVR-Ac.ORF-45Ac.Orf-45
SGP.923pUVR-Ac.ORF-47Ac.Orf-47
SGP.924pUVR-Ac.ORF-48Ac.Orf-48
SGP.925pUVR-Ac.ORF-49proliferating cell nuclear antigen
SGP.927pUVR-Ac.ORF-51Ac.Orf-51
SGP.928pUVR-Ac.ORF-52Ac.Orf-52
SGP.930pUVR-Ac.ORF-53alate expression factor 10
SGP.931pUVR-Ac.ORF-54viral capsid associated protein
SGP.932pUVR-Ac.ORF-55Ac.Orf-55
SGP.933pUVR-Ac.ORF-56Ac.Orf-56
SGP.934pUVR-Ac.ORF-57Ac.Orf-57
SGP.936pUVR-Ac.ORF-59Ac.Orf-59
SGP.938pUVR-Ac.ORF-61Ac.Orf-61
SGP.939pUVR-Ac.ORF-62late expression factor 9
SGP.940pUVR-Ac.ORF-63Ac.Orf-63
SGP.941pUVR-Ac.ORF-64fusolin; spindle body protein
SGP.944pUVR-Ac.ORF-67late expression factor 3
SGP.945pUVR-Ac.ORF-68Ac.Orf-68
SGP.946pUVR-Ac.ORF-69putative methyl transferase
SGP.947pUVR-Ac.ORF-70Ac.Orf-70
SGP.948pUVR-Ac.ORF-71Ac.Orf-71
SGP.949pUVR-Ac.ORF-72Ac.Orf-72
SGP.950pUVR-Ac.ORF-73Ac.Orf-73
SGP.951pUVR-Ac.ORF-74Ac.Orf-74
SGP.952pUVR-Ac.ORF-75Ac.Orf-75
SGP.953pUVR-Ac.ORF-76Ac.Orf-76
SGP.954pUVR-Ac.ORF-77very late expression factor 1
SGP.956pUVR-Ac.ORF-79Ac.Orf-79
SGP.957pUVR-Ac.ORF-80occlusion-derived virus glycoprotein
SGP.958pUVR-Ac.ORF-81Ac.Orf-81
SGP.959pUVR-Ac.ORF-82telokin-like protein-20
SGP.961pUVR-Ac.ORF-84Ac.Orf-84
SGP.962pUVR-Ac.ORF-85Ac.Orf-85
SGP.964pUVR-Ac.ORF-87p15
SGP.965pUVR-Ac.ORF-88Ac.Orf-88
SGP.966pUVR-Ac.ORF-89major viral capsid protein
SGP.967pUVR-Ac.ORF-90late expression factor 4
SGP.968pUVR-Ac.ORF-91Ac.Orf-91
SGP.969pUVR-Ac.ORF-92Ac.Orf-92
SGP.970pUVR-Ac.ORF-93Ac.Orf-93
SGP.971pUVR-Ac.ORF-94occlusion-derived virus envelope protein
SGP.973pUVR-Ac.ORF-96Ac.Orf-96
SGP.974pUVR-Ac.ORF-97Ac.Orf-97
SGP.975pUVR-Ac.ORF-98Ac.Orf-98
SGP.976pUVR-Ac.ORF-99late expression factor 5
SGP.977pUVR-Ac.ORF-100basic protein, p6.9
SGP.978pUVR-Ac.ORF-101Ac.Orf-101
SGP.979pUVR-Ac.ORF-102Ac.Orf-102
SGP.980pUVR-Ac.ORF-103Ac.Orf-103
SGP.982pUVR-Ac.ORF-105Ac.Orf-105
SGP.983pUVR-Ac.ORF-106Ac.Orf-106
SGP.984pUVR-Ac.ORF-107Ac.Orf-107
SGP.985pUVR-Ac.ORF-108Ac.Orf-108
SGP.986pUVR-Ac.ORF-109Ac.Orf-109
SGP.987pUVR-Ac.ORF-110Ac.Orf-110
SGP.988pUVR-Ac.ORF-111Ac.Orf-111
SGP.989pUVR-Ac.ORF-112Ac.Orf-112
SGP.991pUVR-Ac.ORF-114Ac.Orf-114
SGP.992pUVR-Ac.ORF-115Ac.Orf-115
SGP.993pUVR-Ac.ORF-116Ac.Orf-116
SGP.994pUVR-Ac.ORF-117Ac.Orf-117
SGP.995pUVR-Ac.ORF-118Ac.Orf-118
SGP.996pUVR-Ac.ORF-119Ac.Orf-119
SGP.997pUVR-Ac.ORF-120Ac.Orf-120
SGP.998pUVR-Ac.ORF-121Ac.Orf-121
SGP.999pUVR-Ac.ORF-122Ac.Orf-122
SGP.1000pUVR-Ac.ORF-123Ac.Orf-123
SGP.1001pUVR-Ac.ORF-124Ac.Orf-124
SGP.1002pUVR-Ac.ORF-125late expression factor 7
SGP.1003pUVR-Ac.ORF-126Chitinase
SGP.1004pUVR-Ac.ORF-127viral cathepsin-like protein
SGP.1005pUVR-Ac.ORF-128major budded virus envelope glycoprotein
SGP.1006pUVR-Ac.ORF-129viral capsid protein
SGP.1007pUVR-Ac.ORF-130Ac.Orf-130
SGP.1008pUVR-Ac.ORF-131major polyhedral calyx protein, pp34
SGP.1010pUVR-Ac.ORF-133alkaline exonuclease
SGP.1012pUVR-Ac.ORF-135annihilator, 35K
SGP.1013pUVR-Ac.ORF-136Ac.Orf-136
SGP.1014pUVR-Ac.ORF-137fibrous body protein
SGP.1016pUVR-Ac.ORF-139DNA synthesis regulator, ME53
SGP.1017pUVR-Ac.ORF-140Ac.Orf-140
SGP.1019pUVR-Ac.ORF-142early 49 Daa protein
SGP.1020pUVR-Ac.ORF-143occlusion-derived virus envelope protein, E18
SGP.1021pUVR-Ac.ORF-144occlusion-derived virus envelope/capsid protein, EC27
SGP.1022pUVR-Ac.ORF-145Ac.Orf-145
SGP.1023pUVR-Ac.ORF-146Ac.Orf-146
SGP.1024pUVR-Ac.ORF-147early gene transactivator
SGP.1025pUVR-Ac.ORF-148occlusion-derived virus envelope protein, E56
SGP.1026pUVR-Ac.ORF-149Ac.Orf-149
SGP.1027pUVR-Ac.ORF-150Ac.Orf-150
SGP.1028pUVR-Ac.ORF-151early gene transactivator, IE-2
SGP.1029pUVR-Ac.ORF-152Ac.Orf-152
SGP.1030pUVR-Ac.ORF-153Ac.Orf-153

Vector features:

1. Clone genes inframe with His-TEV codons.
2. MCS with BamHI, HindIII restriction sites.

Constructs made with pST2 vector:
Clone ID Construct Name Gene Product
VE.51pSCST-01KSthree-finger_toxin_clone
VE.52pSCST-02KSCobrotoxin-b
VE.53pSCST-03KSweak neurotoxin NNAM1
VE.54pSCST-04KSTryptophan-containing weak neurotoxin
VE.55pSCST-05KSCobrotoxin
VE.56pSCST-06KSNTL-2 Neurotoxin-like protein
VE.57pSCST-07KSCardiotoxin-like basic protein 3
VE.58pSCST-08KSCytotoxin 11
VE.59pSCST-09KSCardiotoxin-2a
VE.60pSCST-10KSCytotoxin 14b
VE.61pSCST-11KSCytotoxin 14a
VE.62pSCST-12KSlong chain neurotoxin 7
VE.63pSCST-13KSMuscarinic toxin-like protein 2
VE.64pSCST-14KSProbable weak neurotoxin NNAM1
VE.65pSCST-15KS Cytotoxin 13
VE.66pSCST-16KSCytotoxin 14c
VE.67pSCST-17KSCardiotoxin 7
VE.68pSCST-18KSNL-1
VE.101pSCST-naja07858L-amino acid oxidase
VE.102pSCST-naja16037Protein disulfide-isomerase TMX3-like
VE.103pSCST-naja22582PDIA4 Protein disulfide-isomerase A4
VE.104pSCST-naja33650Protein disulfide-isomerase (Fragment)
VE.105pSCST-naja09620P4HB Protein disulfide-isomerase
VE.114pSCST-naja39246PA2A2 Acidic Phospholipase A2 (C-term His tag)
VE.110pSCST-naja07834Ohanin
VE.111pSCST-naja13193Kunitz-type serine protease inhibitor NACI
VE.113pSCST-naja30954Kunitz-type protease inhibitor 2
VE.112pSCST-naja28326Hemorrhagic metalloproteinase-disintegrin-like kaouthiagin

Pricing specifications

Cat ID#Service Description Pricing in USD
RC001Cloning <1kb insert220
RC002Cloning <2kb insert290
RC003Cloning <3kb insert360
RC004Cloning <4kb insert430
RC005Cloning <5kb insert500
MG001Mutate <1kb insert240
MG002Mutate <2kb insert310
MG003Mutate <3kb insert380
MG004Mutate <4kb insert450
MG005Mutate <5kb insert520
EV001Clone <1kb gene in E.coli vector260
EV002Clone <2kb gene in E.coli vector330
EV003Clone <3kb gene in E.coli vector400
GC001Cloning of gRNA70
DP003Maxiprep DNA35

*Shipping charges additional, as actuals

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