Molecular Biology Services
The molecular cloning team of MedGenome offers customized plasmid cloning services, and provides the finished ready-to-use DNA constructs. Currently, our service cater to the following molecular cloning needs
Regular PCR-Insert Cloning
Multi-DNA fragments Assembly and Cloning
Cloning in E. coli expression vectors
Customers can choose from the varied promoter types and fusion tags; and get their target genes cloned into these engineered vectors (refer the schematic representation image). These clones are ideal for protein over-expression studies in E. coli.
Cloning of CRISPR-gRNA constructs and HR donor vectors
Design of gRNAs, synthesis and cloning into any preferred vector of the customer. Design and clone the homology arms into donor plasmid for the knock-out, knock-in, and other CRISPR-Cas9 gene-editing modifications.