Title: Identify therapeutic candidates and biomarkers using Immune profiling
Day: 20th - 22nd November, 2019
Title: Multiple Platforms for Single Cell Genomics to Enable Biomarker Discovery in Immunotherapy
Single cell genomic approaches can provide valuable insights into the complexity and heterogeneity of the cell types in the context of a tissue or tumor. However, challenges with the preparation of single cell suspensions, good cell viability and efficiently capturing diverse cell types in a mix via appropriate cell capture methods can override the utility of the approaches. We provide validation and application data generated from a range of single cell input types (number of starting cells, viability, and research question) and diverse commercially available platforms (the 10x Genomics Chromium and the FACS based SMART-Seq (Takara Bio).